Fig. 2
Adhesion and migration of microglia induced by Aβ fibrils were decreased by Nogo-P4. The effects of Nogo on the adhesion and migration of microglia isolated from WT and APP/PS1 mice in different ages to fAβ were measured. a, b Nogo-P4 inhibited adhesion of microglia to Aβ fibrils. Microglia isolated from neonatal, 3-month-old, 15-month-old WT mice or 3-month-old, and 15-month-old APP/PS1 mice were plated on spots of BSA (0.01% in PBS), Rtn-P4 (100 μg/ml), Nogo-P4 (100 μg/ml), fAβ1–42 (10 μM), Rtn-P4 (100 μg/ml) + fAβ1–42 (10 μM), or Nogo-P4 (100 μg/ml) + fAβ1–42 (10 μM) for 24 h. The numbers of cells within spot areas were quantified. a WT mice. b APP/PS1 mice. c, d Nogo-P4 inhibited the migration of microglia to Aβ fibrils. Microglia isolated from neonatal, 3-month-old, 15-month-old WT mice or 3-month-old, and 15-month-old APP/PS1 mice were added to transwell membranes coated on their underside with BSA (0.01% in PBS), Rtn-P4 (100 μg/ml), Nogo-P4 (100 μg/ml), fAβ1–42 (10 μM), Rtn-P4 (100 μg/ml) + fAβ1–42 (10 μM), or Nogo-P4 (100 μg/ml) + fAβ1–42 (10 μM) for 24 h. The numbers of cells that transmigrated through the transwell membranes were quantified. c WT mice. d APP/PS1 mice. Values were reported as the mean ± SD, as a percentage of values determined in BSA group of neonatal mice (control, 100%). **p < 0.01, ***p < 0.001, when compared with the BSA group; ###p < 0.001, when compared with fAβ1–42 group; $p < 0.05, $$p < 0.01, $$$p < 0.001, n = 3