Fig. 2

Effects of 2-AG on the change of cell apoptosis and viability of astrocytes induced by LPS. Astrocytes were pretreated with 0.01 μM 2-AG for 2 h and exposed to 1 μg/ml LPS for 12 h. Cell apoptosis was measured by Hoechst 33342 assay (a, b) (the red arrows indicate the apoptotic cells) and MTT was used to measure cell viability (c). The expressions of Bcl-xl and Bax were evaluated through western blotting assay (d, e). Scar bar = 20 μm, error bars were ± SD. n = 3. *p < 0.05, **p < 0.01, and ***p < 0.001