Fig. 1

Experimental design. The first hrIL-1Ra (50–200 mg/kg) or saline injection was administrated 30 min before the ip injection of LPS from Escherichia coli (50 μg/kg) in pups at P12. Four hours later, the right common carotid artery was ligated, and hypoxia was induced (8% O₂ for 1.5 h). Rat pups were subjected or not to hrIL-1Ra (50–200 mg/kg q12 h from P12 to P14) and treated or not by HT (32.5 °C ± 0.5 °C for 4 h). Abbreviations: HI, hypoxia-ischemia; HT, hypothermia; hrIL-1Ra, human recombinant of interleukin-1 receptor antagonist; ip, intraperitoneally; LPS, lipopolysaccharide from Escherichia coli; P, postnatal day