Fig. 4
Distribution of GFP-expressing neuritogenic T cells in the sciatic nerve after AT-EAN. Histological analysis of distribution of GFP-expressing neuritogenic T cells after adoptive transfer of CD4P2-GFP at different time points in the sciatic nerve. a Cross sections stained with antibodies against either CD4 (upper panel) or GFP (lower panel). Cell nuclei were visualized using DAPI. Representative images of days two and four both showed endoneurium and epineurium. Note the massive cell infiltration in the epineurium, while no cells are detectable in the endoneurium. Endoneurium is visualized for day six. b Higher magnification of GFP+ cell in the epineurium at day four. c Overview of cross sections stained with antibodies against CD4. Representative image of a rare example where CD4 T cells (and GFP+ T cells, data not shown) are detectable in the endoneurium at day four. d Representative longitudinal sections of a proximal region stained with a CD4 antibody (upper panel) or a distal region with GFP antibody (lower panel) at day six. Cell nuclei were visualized using DAPI. Note the patchy cell infiltration in the endoneurium