Fig. 6

HBHP inhibited microglia activation in tPA-treated MCAO rats. A Representative graphs of immunofluorescence staining with anti-Iba-1 antibodies (green) and DAPI (blue) in the peri-infarct cortex from 4.5 h MCAO rats. Scale bar = 50 μm. B Representative graphs of immunohistochemistry staining with CD68 antibodies. The volumes of e–h were the amplification of a–d. Scale bar = 500 μm in a–d (4×); Scale bar = 50 μm in e–h (40×). C The number of Iba-1- and CD68-positive cells was quantified. D 2-3-5-Triphenyl tetrazolium chloride staining of ischemic brain indicated the position where the corresponding images were obtained. The brain region surrounded by black solid line indicates the areas where the typical Iba-1-positive cells were observed. *P < 0.05, **P < 0.01; n = 3–4. Data were shown as mean ± SD