Fig. 5

Activated microglia within white matter tracts: At 3 months of age, OX-6 positive activated microglia were sparse/rare within the corpus callosum and internal capsule (a). At 15 months of age, significantly more OX-6 positive signal was found within the corpus callosum of TG than WT rats (b). At 19 months of age, there were no statistical significant differences between activated microglia within the corpus callosum or internal capsule between WT and TG rats (c). The amount of OX-6 positive microglia in both white matter tracts increased visibly at 15 months, especially in TG rats (b). Activation of microglia in white matter continues to increase to 19 months of age, with comparable amounts in both genotypes (c). Quantification of OX-6 positive activated microglia within the white matter tracts showed a significantly higher load in TG vs. WT rats at 15 months, but not at 19 months in both the corpus callosum and (d) and internal capsule (e). Data is represented as group mean ± SEM. An asterisk indicates statistical significance between WT and TG rats, the number sign indicates statistical significance between 15 and 19 months WT or TG rats, respectively (p < 0.05, two-way ANOVA, Tukey’s post hoc test, n values indicated within bars on graphs)