Fig. 5

Anti-TLR2 treatment triggers oxidative phosphorylation. Primary microglia were prepared for metabolic analysis as described in the legend to Fig. 4 and in the “Methods” section. a The OCR bioenergetics profile consisting of three baseline measures of OCR followed by sequential measures following exposure to oligomycin (20 μM), carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (20 μM; FCCP) and antimycin A (40 μM) is shown. b–e LPS + Aβ exerted no significant effect on mean basal respiration b, mean maximal respiration (c) or mean ATP production (d) or mean proton leak (e) but, except for maximal respiration and proton leak, each measure was significantly increased when cells were incubated in LPS + Aβ+anti-TLR2 antibody (***p < 0.001, *p < 0.05, ⁺p < 0.05; LPS + Aβ vs LPS + Aβ+anti-TLR2 antibody; (F3,16) = 9.666; p = 0.0010; one-way ANOVA, Newman-Keuls multiple comparison test); three independent experiments were performed