Fig. 6

Peripheral inflammation increases in ITGB4^−/− mice. a H & E staining of mouse lung tissues from the control group and the ITGB4^−/− group. All images were obtained at × 200 (upper) and × 400 (lower) magnification. b Lung tissues Th1 cells (%), Th2 cells (%) and T17 cells (%) determined by flow cytometry in mice from the control group and the ITGB4^−/− group. Th2 cells in ITGB4^−/− group showed a significant increase. c, d Measurements of IL-4 levels (c) and IL-13 levels (d) in the BLAF and serum by ELISA. The IL-4 and IL-13 in ITGB4^−/− group showed significant increase compared to that in the control group. The data are expressed as the mean ± SEM of four mice in each group. *p < 0.05, **p < 0.01