Fig. 8

Central nervous inflammation enhanced in ITGB4^−/− mice. a, b IBA1 and TNFRα proteins expression in the hippocampus (a) and prefrontal cortex (b) determined and quantified by western blot against the expression of β-actin. The ITGB4^−/− mice demonstrated significantly higher levels of IBA1 and TNFRα than in the control. c, d, e Immunohistochemical staining was performed to assess the expression of TNFα (c), IL-1B (d) and IL-6 (e) in the hippocampus. All images were obtained at × 200 (upper) and × 400 (lower) magnification. f, g, h Immunohistochemical staining was performed to assess the expression of TNFα (f), IL-1β (g) and IL-6 (h) in the prefrontal cortex. All images were obtained at × 200 (upper) and × 400 (lower) magnification. All data are presented as mean ± SEM of four mice in each group. ***p < 0.001