Fig. 2

Effect of minocycline on NTG-induced mechanical hypersensitivity and microglia response. Prior to NTG/saline administration, mice were treated with vehicle or minocycline (Mino, 30 mg/kg, i.p.) every other day for 9 days. a Chronic treatment with minocycline significantly altered basal hypersensitivity. p < 0.01 for drug, time, and interaction. Two-way RM ANOVA and Bonferroni post hoc analysis. n = 8/group. ***p < 0.001 compared to the VEH-VEH group. b Post-treatment responses were assessed 2 h following NTG administration. Minocycline did not prevent acute NTG-induced hyperalgesia. p < 0.001 effect of drug, but no significant effect of time or interaction, two-way RM ANOVA. n = 8/group. c Immunostaining of the TNC for Iba1 in the NTG group and the NTG + Mino group on day 9; scale bar, 50 μm. d Quantitative analysis of the number of Iba1-positive cells. e The ratio of area immunoreactive for Iba1 to the total area of the ipsilateral TNC. Unpaired t test, n = 6/group. ***p < 0.001