Fig. 1

Genetic knock-out of CIITA reduces α-syn-induced inflammation and antigen processing in vitro. a WT and CIITA −/− primary microglial cultures were treated with α-syn monomer or α-syn fibrils (200 ng/mL) for 2 h and immunolabeled with iNOS (red) and MHCII (green, far right panel). iNOS scale bar represents 25 μm, and MHCII and iNOS image scale bar represents 10 μm. b Representative images of WT or CIITA −/− cells treated with DQ-Ovalbumin (green) to assess antigen processing. Cells were treated with α-syn monomer or α-syn fibrils at 200 ng/mL for 2 h, or IFNy (10 ng/mL) as a positive control. Scale bar represents 25 μm. c Quantification (fold change over WT microglia α-syn monomer treated control) of iNOS expression in panel a. Each group was comprised of 16 individual samples, with four images quantified per sample. Two-way ANOVA with Tukey’s multiple comparisons, ****p < 0.0001. d Quantification of DQ-Ovalbumin experiments in b. Each experimental group was comprised of eight individual samples, with four images quantified per sample. Samples were normalized to WT microglia α-syn monomer treated control. Two-way ANOVA with Bonferroni’s multiple comparisons, *p < 0.05, ****p < 0.0001