Fig. 3

NOD2 deficiency attenuated dopaminergic degeneration induced by 6-OHDA. Wild-type (WT) and NOD2-deficient (NOD2^−/−) mice were placed in a stereotaxic device under 1.5% pentobarbital sodium anesthesia and given 6-OHDA or saline alone into two different sites of the STR separately. a The levels of dopamine and its metabolites in the striatum were measured by HPLC/MS analysis on 14 days after 6-OHDA injection. b The brain sections from the SN and STR showing TH immunoreactivity on 14 days after striatal infusion of 6-OHDA. NOD2 deficiency improved the reduction of tyrosine hydroxylase (TH) protein expression in the SN (c) and STR (d) on 14 days after 6-OHDA injection. All data are presented as the mean ± SEM, n = 6 mice per group. *p < 0.05, compared with indicated group