Fig. 6

Mrp8/14-induced BV2 microglia activation was alleviated by a TLR4 inhibitor (TAK-242). Cells were administrated with TAK-242 for 30 min before treatment of Mrp8/14 heterodimer. a The iNOS protein level was reduced in Mrp8/14 + TAK-242 group compared with Mrp8/14 group (n = 4 per group). b The iNOS mRNA level was reduced in Mrp8/14 + TAK-242 group compared with Mrp8/14 group (n = 3–4 per group). c The production of nitric oxide (NO) induced by Mrp8/14 was decreased when pretreatment of TAK-242 (n = 3–4 per group). d The representative photos exhibited that the production of reactive oxygen species (ROS) induced by Mrp8/14 was reduced when pretreatment of TAK-242. *p < 0.05, **p < 0.01, ***p < 0.001 between two indicated groups