Fig. 11

Ibrutinib significantly downregulated LPS-stimulated COX-2 levels in vivo. a Wild-type mice were injected with ibrutinib (10 mg/kg, i.p.) or vehicle (10% DMSO, i.p.) daily for 3 days, followed by injection of LPS (10 mg/kg, i.p.) or PBS. Three hours after the injection of LPS or PBS, the mice were perfused, fixed, and immunostained with an anti-COX-2 antibody in the hippocampus. b, c Quantification of the data from a (hippocampus: con, n = 4 mice; LPS, n = 4 mice; ibrutinib+LPS, n = 4 mice). d Wild-type mice were injected with ibrutinib (10 mg/kg, i.p.) or vehicle (10% DMSO, i.p.) daily for 3 days, followed by injection with LPS (10 mg/kg, i.p.) or PBS. Three hours after the injection of LPS or PBS, the mice were perfused, fixed, and immunostained with an anti-COX-2 antibody in the cortex. e Quantification of the data from d (cortex: con, n = 4 mice; LPS, n = 4 mice; ibrutinib+LPS, n = 4 mice). **p < 0.01, ***p < 0.001