Fig. 2

Human tau was quantified by Simoa (single molecule array) assay and is expressed here as fold-change from WTs. a Experimental design. b Lysate from dissociated cells depleted of microglia from rTg4510 mice contain high levels of human tau, significantly more than WT littermates (p < 0.001). c Microglia isolated from rTg4510 mice contain human tau protein on DIV3 by two-tailed t test (p = 0.03) even though they do not express the human tau gene MAPT (Fig. 1). This expression tapers off over time (p < 0.05 on DIV6 and DIV9). However, a two-way ANOVA did not reveal any main effects of genotype or timepoint. c Conditioned media from rTg4510 microglia contain significantly more tau than conditioned media from WT littermates on DIV3 and DIV6 (p < 0.001) and on DIV9 (p < 0.01) as measured by a two-way ANOVA with a Bonferroni post-hoc test. *** p < 0.001; ** p < 0.05, and ~p < 0.05 by t test at the given timepoint but not by ANOVA