Fig. 4
From: The role of microglia in processing and spreading of bioactive tau seeds in Alzheimer’s disease
Tau seeds released by microglia in their condition media were quantified by a FRET-based biosensor cell line that detects tau aggregates. rTg4510, rTg21221, and WT microglia-conditioned media was collected on DIV3, DIV6, DIV9, and DIV12, applied to the seeding assay, and quantified by flow cytometry after 72 h. Analysis by two-way ANOVA demonstrated a significant main effect of genotype (p < 0.0001) but not DIV (p = 0.3674). Bonferonni post-test revealed that the rTg4510 microglia CM induced significantly more seeding than the rTg21221 or WT microglia CM on DIV3 (p < 0.01 and p < 0.001, respectively) and more than the WT microglia on DIV6 (p < 0.05). While there was still some FRET seeding activity on DIV9 and DIV12, it was not significantly higher than either the rTg21221 or WT microglia CM (Fig. 4)