Fig. 6
ZIKV infection activates UPR downstream genes in the mouse brain. Three-week-old AG6 mice were infected with 1 × 105 PFU/mouse (each dose, N = 3) via intraperitoneal injection. The total RNA was extracted from the nervous tissues of the mouse brains. Following the synthesis of the first strand cDNA, RT-qPCR assay was performed to assess the transcriptional levels of downstream genes of ER stress, namely, atf4, gadd34, chop, and edem-1. The relative expression levels of genes were calculated according to the 2−∆∆Ct method. Data represent three independent experiments and error bars indicate mean ± SD. Statistical analyses were performed using multiple t tests (N = 3) (P < 0.05 or P < 0.01)