Fig. 7

ZIKV infection activates the UPR downstream genes in human neural cells. a–h CCF-STTG1 and SK-N-SH were infected with ZIKV at an MOI of 5 PFU/cell. RNA was extracted at 24 and 48 hpi and the first strand cDNA was synthesized. Primers that specifically amplified atf4 (a, b), gadd34 (c, d), chop (e, f), and edem-1 (g, h) were used. The relative expression levels of the genes were calculated according to the 2^−∆∆Ct method. Data represented three independent experiments and error bars indicate mean ± SD. Statistical analyses were performed using multiple t tests (N = 3) (P < 0.05 or P < 0.01)