Fig. 6
From: α1-antitrypsin mitigates NLRP3-inflammasome activation in amyloid β1–42-stimulated murine astrocytes
A1AT time-dependently blocked Aβ1–42-induced upregulation of caspase 1 mRNA in primary astrocytes. (b–c) As quantified by RT-PCR treatment with 4 μM Aβ1–42 significantly increased mRNA levels of caspase 1 in astrocytes at 6 h, but not at 3 h. Co-treatment with A1AT blocked this increase in caspase 1 mRNA expression significantly at 6 h, but not at 3 h. Addition of MCC950 had no effects on caspase 1 mRNA expression at both stimulation times. Data of n = 3 in triplicate represent mean ± SD.*/ap < 0.05; **/aap < 0.01; ***/aaap < 0.001, ns not significant compared to untreated cell control (ctr)