Fig. 3

Pretreatment with LPS followed by CA140 treatment decreased LPS-induced proinflammatory cytokine levels in mouse primary microglial cells. a–f Mouse primary microglial cells were pretreated with LPS (1 μg/mL) or PBS for 30 min, followed by treatment with vehicle (1% DMSO) or CA140 (10 μM) for 5.5 h. Total RNA was then isolated, and proinflammatory cytokine levels were measured by RT-PCR (con, n = 4; LPS, n = 4; LPS + CA140, n = 4). g–l Mouse primary astrocytes were pretreated with LPS (1 μg/mL) or PBS for 30 min, followed by treatment with vehicle (1% DMSO) or CA140 (10 μM) for 5.5 h. Total RNA was then isolated, and proinflammatory cytokine levels were measured by RT-PCR (COX-2: con, n = 20; LPS, n = 20; LPS + CA140, n = 20; IL-1β: con, n = 20; LPS, n = 20; LPS + CA140, n = 20; IL-6, iNOS, and TNF-alpha: con, n = 16; LPS, n = 16; LPS + CA140, n = 16). *p < 0.05, **p < 0.001, ***p < 0.0001