Fig. 11

LIF treatment decreases IL-12 p40 release and increases IL-10 release in pro-inflammatory BMDMs. A pro-inflammatory (M1) phenotype was induced in BMDMs via stimulation with IFNγ and LPS. LIF or PBS were co-administered with stimulants. a At 24 h after stimulation, macrophage-conditioned media from M1 cells treated with LIF had significantly lower IL-12 p40 release compared to media from M1 cells treated with PBS (**p < 0.01). b IL-10 release in macrophage-conditioned media from M1 cells treated with LIF compared to M1 cells treated with PBS (*p < 0.05). c The average ratio of IL-12 p40/IL-10 in the macrophage-conditioned media from LIF-treated M1 cells was significantly lower than the IL-12 p40/IL-10 ratio in the PBS-treated M1 cells (**p < 0.01). n = 3 wells per treatment group