Fig. 5

Global brain immune changes return to baseline after 18 weeks of high-fat feeding and/or hyperglycemia. a Flow cytometry analysis of brain CD45⁺7AAD⁻ leukocytes of the left cerebral hemisphere. b Gated cells were analysed for the expression of CD11b as a marker of neutrophils, monocytes and microglia. c CD11b⁺ cell gate was further analysed for expression of CD68 as a phagocytic marker. d Gated cells for B220 as a marker of B cells and (e) CD3 as a marker of T cells were analysed. f Number of Iba1⁺ microglia and (g) GFAP⁺ astrocytes in CA1 of the hippocampus were quantified at 20 weeks, and (h) microglial and (i) astrocyte numbers were compared between time points in CIT + CON mice. Data are shown as mean ± S.E.M. of a–e, n = 8–10 per group. Data in a–c analysed by one-way ANOVA with Dunnett’s. d and e analysed by Kruskal-Wallis with Dunn’s. h and i by Student’s t test. *P < 0.05, ****P < 0.0001 vs CIT + CON control group