Fig. 1
From: Complement-dependent bystander injury to neurons in AQP4-IgG seropositive neuromyelitis optica
Characterization of rat astrocyte-neuron cocultures. a GFAP and MAP2 immunofluorescence of pure neuron cultures, pure astrocyte cultures, and astrocyte-neuron cocultures at indicated cell ratios. b (top panels) GFAP or MAP2 immunofluorescence (with DAPI counterstaining) of pure AQP4+/+ and AQP4−/− astrocyte cultures and pure neuron cultures. (lower panels) CD59 and AQP4 immunofluorescence. Where indicated (bottom row), cells were incubated with PI-PLC 1 h prior to fixation. c CD59 immunofluorescence of astrocyte-neuron cocultures costained with GFAP or MAP2. d (upper) AQP4 immunofluorescence of astrocyte-neuron cocultures costained with GFAP and MAP2. (lower) AQP4-IgG (human IgG, hIgG) immunofluorescence (visualized with secondary anti-human IgG antibody) in astrocyte-neuron cocultures following 1 h incubation with 20 μg/ml AQP4-IgG, costained with AQP4 and MAP2. Micrographs representative of studies done of three sets of cultures