Fig. 6

Evidence for complement bystander killing of neurons in rat brain. a Brains were injected with AQP4-IgG and EH-1 and harvested at 90 min as in Fig. 5. High-magnification confocal micrographs showing colocalization of EH-1, GFAP, and C5b-9 (top, filled arrowheads) and EH-1, NeuN, and C5b-9 (bottom, open arrowheads). b Brains were injected with AQP4-IgG alone (without EH-1) and harvested at 90 min. C1q colocalization with GFAP and NeuN, and C5b-9 colocalization with GFAP and NeuN (bottom). Filled arrowheads show C5b-9 or C1q deposition on astrocytes, and open arrowheads show C5b-9 on neurons. c CD59 immunofluorescence with GFAP or NeuN in control (non-injected) rat. Filled arrowheads indicate CD59 on astrocytes. Representative of micrographs done on sections from three rats