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Table 1 Target genes for the PCR analyses are listed with their corresponding GeneID number and oligonucleotide sequences for the forward and reverse primers

From: TLR4 inhibitor TAK-242 attenuates the adverse neural effects of diet-induced obesity

Target gene Primer sequence
β-actin
Gene ID: 11461
Forward: 5′-AGCCATGTACGTAGCCATCC-3′
Reverse: 5′-CTCTCAGCTGTGGTGGTGAA-3′
β-site APP cleaving enzyme (BACE1)
GeneID: 23821
Forward: 5′-TCGCTGTCTCACAGTCATCC-3′
Reverse: 5′-AACAAACGGACCTTCCACTG-3′
Cluster of differentiation factor 36 (CD36)
GeneID: 12491
Forward: 5′-TATTGGTGCAGTCCTGGCTG-3′
Reverse: 5′-CTGCTGTTCTTTGCCACGTC-3′
Cluster of differentiation factor 68 (CD68)
GeneID: 12514
Forward: 5′-TTCTGCTGTGGAAATGCAAG-3′
Reverse: 5′-AGAGGGGCTGGTAGGTTGAT-3′
Cluster of differentiation factor 74 (CD74), transcript variant 1
GeneID: 16149
Forward: 5′-CAAGTACGGCAACATGACCC-3′
Reverse: 5′-GCACTTGGTCAGTACTTTAGGTG-3′
EGF-like module-containing mucin-like hormone receptor-like 1 (F4/80)
GeneID: 13733
Forward: 5′-TGCATCTAGCAATGGACAGC-3′
Reverse: 5′-GCCTTCTGGATCCATTTGAA-3′
Insulin-degrading enzyme (IDE)
GeneID: 15925
Forward: 5′-TGTTTCCACACACAGGCAAT-3′
Reverse: 5′-ACCTGTGAAAAGCCGAGAGA-3′
Interleukin-1β (IL1β)
GeneID: 16176
Forward: 5′-GCAACTGTTCCTGAACTCAACT-3′
Reverse: 5′-ATCTTTTGGGGTCCGTCAACT-3′
Interleukin-6 (IL6)
GeneID:16193
Forward: 5′-CTCTGGGAAATCGTGGAAAT-3′
Reverse: 5′-CCAGTTTGGTAGCATCCATC-3′
Lipoprotein lipase (LPL)
GeneID: 16956
Forward: 5′-GGGCCCAGCAACATTATCCA-3′
Reverse: 5′-GGGGGCTTCTGCATACTCAA-3′
Major histocompatibility complex class II (MHC II)
GeneID: 14961
Forward: 5′-CAGACGCCGAGTACTGGAAC-3′
Reverse: 5′-CAGCGCACTTTGATCTTGGC-3′
Neprilysin
GeneID: 17380
Forward: 5′-GAGAAAAGCCCACTTGCTTG-3′
Reverse: 5′-GAAAGACAAAATGGGGCAGA-3′
Phosphoglycerate kinase 1 (Pgk1)
GeneID: 18655
Forward: 5′-GCCTGTTGACTTTGTCACTGC-3′
Reverse: 5′-GAGTGACTTGGTTCCCCTGG-3′
Succinate dehydrogenase complex, subunit A, flavoprotein (SDHA)
Gene ID: 66945
Forward: 5′-ACACAGACCTGGTGGAGACC-3′
Reverse: 5′-GGATGGGCTTGGAGTAATCA-3′
Tumor necrosis factor α (TNFα), transcript variant 1
GeneID: 21926
Forward: 5′-CCCTCACACTCAGATCATCTTCT-3′
Reverse: 5′-GCTACGACGTGGGCTACAG-5′