Fig. 6

Euthyroid HT induces microglia and astroglia activation in the frontal cortex. IHC and real-time RT-PCR were performed to assess the activation state of microglia and astrocytes in animals. a, b Representative images of Iba1 (microglia marker) and GFAP (astrocyte marker) staining in the frontal cortex. Each right-hand panel (× 400) depicts a magnified image of the boxed area of the corresponding image in the left panel (× 100). Black arrows indicate resting microglia or astroglia. Red arrows indicate activated microglia or astroglia. c Quantitative analysis of Iba1 immunoreactivity. d Quantitative analysis of GFAP immunoreactivity. e PCR analysis of Iba1 and GFAP. Data are presented as the mean ± SEM, n = 5; ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001, vs. Con