Fig. 7

Knockdown of lncRNA AK006025 reduced CXCL9/10/11 cluster gene expression in astrocytes treated with Nef. a Real-time PCR assay detection of lncRNA expression in astrocytes transfected siRNAs for 48 h, followed by treatment with Nef protein for 6 h and 12 h. b Real-time PCR assay analysis of CXCL9, CXCL10, and CXCL11 mRNA transcriptional level in astrocytes transfected siRNAs for 48 h, followed by treatment with Nef protein for 12 h. c ELISA analysis of CXCL9, CXCL10, and CXCL11 secretion in astrocytes transfected siRNAs for 48 h, followed by treatment with Nef protein for 24 h. *P < 0.05, **P < 0.01, ***P < 0.001, vs 0 h. Error bars represent means ± SEM. These data are from three independent experiments