Fig. 4

KCa3.1 contributes to increased ER stress in APP/PS1 mice. a Western blot analysis of GRP78 and CHOP protein levels in hippocampal extracts of 15-month-old WT, KCa3.1^−/−, APP/PS1, and KCa3.1^−/−/APP/PS1 mice. b, c Data are presented as the mean ± SEM (n = 3–5). The OD values of GRP78 (b) and CHOP (c) were normalized to that of β-actin. #p < 0.05 vs. WT, *p < 0.05 vs. APP/PS1 (one-way ANOVA followed by the Dunnett’s multiple comparison test). d Double immunofluorescence staining of GFAP with GRP78 in CA1 areas of the mouse hippocampus. DAPI (blue) was used to label nuclei. e Quantification of the percentage of GRP78⁺ cells colabeled with GFAP. **p < 0.01 vs. control group (one-way ANOVA followed by the Dunnett’s multiple comparison test) (n = 4). Scale bar: 25 μm. WT wild-type