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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Anti-inflammatory and anti-oxidant mechanisms of an MMP-8 inhibitor in lipoteichoic acid-stimulated rat primary astrocytes: involvement of NF-κB, Nrf2, and PPAR-γ signaling pathways

Fig. 5

M8I inhibited ROS production and increased antioxidant enzyme expression. a, b Effect of M8I on ROS production in a LTA or b H2O2-stimulated astrocytes. Cells were pretreated with M8I for 1 h, followed by treatment with LTA (10 μg/ml) or H2O2 (500 μM) for 30 min. Intracellular ROS levels were measured by the DCF-DA method. c Western blot analysis for phosphorylation of the p47phox subunit of NADPH oxidase. Cells were pre-treated with M8I for 1 h, followed by LTA (10 μg/ml, for 15 min), and then subjected to immunoblot analysis using antibodies against phospho-p47phox. Quantification data are shown in the graph (n = 3). d Effect of M8I on the mRNA expression of HO-1, NQO1, catalase, and MnSOD. e Quantification of RT-PCR data (n = 3). f Effect of M8I on the protein expression of HO-1, NQO1, catalase, and MnSOD. g Quantification of Western blot analysis (n = 3). #P < 0.05, vs. control samples. *P < 0.05, vs. LTA-treated samples

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Journal of Neuroinflammation

ISSN: 1742-2094