Fig. 4

Overexpression of βCaMKII in CA1 regions produces depression-like behaviors in wild-type rats. a Schematics of AAV vectors engineered to overexpress a control construct or βCaMKII. ITR, inverted terminal repeats; EF1, ZsGreen promoter; CMV, βCaMKII promoter. b Experimental paradigm for behavioral and biochemical testing of rats infected with the virus. c Illustration of viral infusion of AAV-βCaMKII into the CA1 region. Scale bar is 200 μm. d, e Behavioral effects after overexpressing AAV-βCaMKII in the CA1. N = 18 per group. Data are presented as the means ± SEM. *P < 0.05, **P < 0.01 WT+AAV-βCaMKII vs WT; ^#P < 0.05, ^##P < 0.01 WT+AAV-βCaMKII vs WT+AAV-control (WT, wild type)