Fig. 2

Tissue damage and functional changes following photothrombotic stroke. a Cresyl-violet stained coronal section of a rat brain at 24 h after induction of photothrombotic stroke showing the clearly defined infarct and the ROIs used for analysis of sections immunolabeled to detect Iba1. The scale is in millimeters. b Cresyl-violet stained coronal section at 3 h after stroke induction. c Infarct volume over 7 days after stroke induction. The infarct is fully developed by 24 h and then contracts substantially by 7 days (n = 4–6 per time point). d Coronal section of cerebral cortex immunolabeled for NeuN at 3 h after stroke induction (scale bar = 500 μm). e Box plot of forelimb placing scores following stroke. Outlier values are shown as circles; Blue = forelimb ipsilateral to infarct, Red = forelimb contralateral to infarct (n ≥ 5 per group)