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Table 1 Description of statistical analyses

From: Peripheral adaptive immunity of the triple transgenic mouse model of Alzheimer’s disease

Figure referenced Statistical analysis P value Differences between means or mediansa
1A Unpaired t test P > 0.05  
1B (except short-term reconstituting stem cells) Unpaired t test P > 0.05  
1B—short-term reconstituting stem cells Mann-Whitney test P = 0.041 Median A = 0.155; Median B = 0.075; Difference (Hodges-Lehmann): − 0.06
3A—serum IgG Mann-Whitney test P < 0.0001 Median A = 0.77; Median B = 4.505; Difference (Hodges-Lehmann): 3.33
3A—cortical IgG Mann-Whitney test P = 0.0002 Median A = 0.4287; Median B = 0.9211; Difference (Hodges-Lehmann): 0.5244
3A—IgG-secreting B lymphocytes Unpaired t test P = 0.059  
3A—Plasma cells Mann-Whitney test P = 0.041 Median A = 1.09; Median B = 1.505; Difference (Hodges-Lehmann): 0.385
4—IL-1β, IL-3, MCP-1, RANTES Two-way ANOVA P > 0.05  
4—IL-1α, IL-4, IL-5, IL-6, IL-10, IL-12p70, MIP-1α Kruskal-Wallis test
Mann-Whitney test (NTg vs. 3xTg-AD)
P > 0.05
P > 0.05
 
4—IL-2 Kruskal-Wallis test   Not applicable
Dunn’s multiple comparison: P = 0.0256
9 months NTg vs. 3xTg-AD P = 0.0816.
13 months NTg vs. 3xTg-AD P = 0.0530
4—IL-2 Mann-Whitney test (NTg vs. 3xTg-AD) P = 0.0018 Median A = 7.5; Median B = 108.7; Difference (Hodges-Lehmann): 84.9
4—IL-17 Two-way ANOVA   Not applicable
Interaction P = 0.8262
Age P = 0.7464
Genotype P = 0.0115
4—GM-CSF Kruskal-Wallis test   Not applicable
Dunn’s multiple comparison: P = 0.0460
9 months NTg vs. 3xTg-AD P = 0.0246
13 months NTg vs. 3xTg-AD P = 0.4916
4—GM-CSF Mann-Whitney test (NTg vs. 3xTg-AD) P = 0.0085 Median A = 88.4; Median B = 524; Difference (Hodges-Lehmann): 347
4—TNFα Kruskal-Wallis test   Not applicable
Dunn’s multiple comparison: P = 0.0135
9 months NTg vs. 3xTg-AD P = 0.0179
13 months NTg vs. 3xTg-AD P = 0.3412
4—TNFα Mann-Whitney test (NTg vs. 3xTg-AD) P = 0.0050 Median A = 18.4; Median B = 86.9; Difference (Hodges-Lehmann): 65.2
  1. GM-CSF granulocyte-macrophage colony-stimulating factor, IL Interleukin, MCP-1 monocyte chemoattractant protein-1, NTg non-transgenic animals, RANTES regulated on activated, normal T cell expressed and secreted; TNF-α, tumor necrosis factor α
  2. Parametric tests were used only when normality was verified using D’Agostino & Pearson’s normality test. For cytokine/chemokine analysis (Fig. 4), two-way ANOVA was performed when normality of the residuals was confirmed (IL-1β, IL-3, IL-17, MCP-1, and RANTES). When normality was not confirmed, cytokine/chemokine data were analyzed first using Kruskal-Wallis test followed by Dunn’s multiple comparison. Then to identify the global effect of genotype, results from the two ages were grouped (N = 15 NTg; N = 16 3xTg-AD) and analyzed using Mann-Whitney test. Significant P-values are italicized
  3. aFor comparison between groups; significant data only.