Fig. 2

In vivo morphology analysis of M/Ms during the development of melanoma brain metastases. a Representative fluorescence images of the morphology of M/Ms in both sides on day 1 and day 21 after melanoma cell or PBS injection. Red: RFP, green: EGFP. Scale bar: 10 μm. b Schematic of the soma and processes of ramified or amoeboid M/Ms. c Mean soma diameter of M/Ms after RFP-B16 or PBS injection in the ipsilateral side. n = 15–35 cells at each time points from 6 mice per group. d Mean process length of M/Ms; n = 12–19 cells at each time point from 6 mice per group. e Branching parameter of M/Ms; n = 6 mice per group. The data are presented as the mean ± SEM