Fig. 5

MMP3 expression in M/Ms contributed to their activation and disruption of the tight junction. a Agarose gel electrophoresis results of RT-PCR for detecting the expression of MMPs and the quantitative analysis. WT: C57BL/6 mice. TR: CX3CR1-GFP mice. n = 3 mice per group. b Representative immunofluorescence images of MMP3 in M/Ms. Blue: DAPI, green: EGFP, red: MMP3. Scale bar: 20 μm. c Quantitative analysis of the images shown in (b); n = 10 images from 3 mice per time point. d Correlation analysis between the volume density, branching parameter, soma velocity of M/Ms and the proportion of MMP3⁺ M/Ms in the RFP-B16 injection group; n = 6 mice per group. The data are presented as the mean ± SEM. e Representative immunofluorescence results for ZO-1 expression in the brain. Blue: DAPI, green: EGFP, red: ZO-1. Scale bar: 20 μm. f Quantitative results for the proportion of ZO-1⁺ cells shown in (f); n = 10 images from 3 mice per time point. g Correlation analysis between the proportion of MMP3⁺ M/Ms and the proportion of ZO-1⁺ cells. The data are presented as the mean ± SEM