Fig. 9

Immunofluorescence and qPCR experiments indicate that TRPC6 expression is not altered by TBI. Immunofluorescence images of the aortic arch sections incubated with or without the TRPC6-antibody (green) and the smooth muscle actin antibody (red) are shown in a. Blue color corresponds to the DAPI staining. The aortic arches were isolated from sham or TBI 129S-C57BL/6-F2 mice. A adventitia, SM smooth muscle layer, E endothelium layer, L lumen, × 40 magnification. b A summary for data presented in a. c qRT-PCR was used to measure the expression of TRPC1 and TRPC6 mRNAs, as indicated. Data presented were normalized to an internal control encoding hypoxanthine-guanine phosophoribosyltransferase (Hprt) and are expressed relative to levels in the control sham mice. Relative expression = 2^−ΔΔCt, where ΔΔCt = (Ct_TBI − Ct_Hprt)–(Ct_sham − Ct_Hprt). Each circle represents an individual mouse. The mean ± S.E. is also indicated