Fig. 1

Peripheral chimerism analysis of transplanted mice after different pre-conditioning regimens. a Four groups received different pre-conditioning regimens: (i) non-myeloablative conditioning (NMC) using a single dose of busulfan at 25 mg/kg (n = 6/group), (ii) full conditioning (FC) busulfan 125 mg/kg given over five consecutive days (n = 6/group), (iii) head-shielded irradiation (HIR) at a divided dose of 11 Gy given 3 h apart (n = 6/group) and (iv) untransplanted controls (UnTx) (n = 6/group). b Peripheral blood chimerism at 2 weeks post-transplant, c 4 weeks post-transplant, d 8 weeks post-transplant and e 12 weeks post-transplant. f Bone marrow chimerism measured at 12 weeks post-transplant and g spleen chimerism at 12 weeks. One-way ANOVA with Tukey’s post hoc correction for multiple comparisons was used. Error bars represent the SEM. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns = non-significant