Table 2 Antibodies used to immunophenotype brain samples
Manufacturer | Fluorochrome conjugated | Antibody/antigen | Clone | Isotype | Dilution | Catalogue number |
|---|---|---|---|---|---|---|
Biolegend | AF488 | PDCA-1 | 927 | Rat IgG2b, κ | 1:25 | 127012 |
Biolegend | PerCP/Cy5.5 | CX3CR1 | SA011F11 | Mouse IgG2a, κ | 1:25 | 149009 |
Miltenyi Biotec | APC | MerTK | REA477 | Recombinant human IgG1 | 1:5 | 130-107-479 |
Biolegend | AF700 | Ly6G | 1A8 | Rat IgG2a, κ | 1:15 | 127621 |
eBiosciences | APC/eFluor780 | CD3 | 17A2 | Rat IgG2b, κ | 1:50 | 47-0032-82 |
eBiosciences | APC/eFluor780 | CD19 | eBIO1D3 | Rat IgG2b, κ | 1:75 | 47-0193-82 |
BD | BV421 | Siglec-F | E50-2440 | Rat IgG2a, κ | 1:150 | 562681 |
Biolegend | BV510 | Ly6C | HK1.4 | Rat IgG2c, κ | 1:300 | 128033 |
Biolegend | BV650 | CD45.1 | A20 | Mouse (A.SW) IgG2a, κ | 1:75 | 110735 |
Biolegend | BV711 | CD11b | M1/70 | Rat IgG2b, κ | 1:100 | 101241 |
Life Technologies | Live/dead blue | Cellular protein (amines) | – | – | 1:400 | L23105 |
Biolegend | PE | Siglec-H | 551 | Rat IgG1, κ | 1:25 | 129606 |
Biolegend | PE/CF594 | CD45.2 | 104 | Mouse (SJL) IgG2a, κ | 1:200 | 109845 |
Biolegend | PE/Cy5 | MHCII | M5/114.14.2 | Rat IgG2b, κ | 1:6000 | 107611 |
Biolegend | PE/Cy7 | CD64 | X54-5/7.1 | Mouse IgG1, κ | 1:40 | 139313 |