Fig. 4

Ureaplasma-driven pyroptosis in HBMEC. Key genes in pyroptosis (Fig. 1) were assessed for mRNA responses upon stimulation of HBMEC for 4 h and 30 h. Caspase 1 mRNA expression was determined via RNA sequencing (a) and qRT-PCR (b). Similarly, RNA sequencing (c) and qRT-PCR (d) were used to assess caspase 4 mRNA levels. RNA sequencing furthermore determined mRNA expression of NLRP3 (e) and gasdermin D (f). Data are presented as means ± SD (*p < 0.05, **p < 0.01, ***p < 0.001 vs. unstimulated control; °p < 0.05, °°p < 0.01, °°°p < 0.001 vs. broth; ^#p < 0.05, ^##p < 0.01, ^####p < 0.0001 vs. LPS)