Fig. 3From: Co-inhibition of PGF and VEGF blocks their expression in mononuclear phagocytes and limits neovascularization and leakage in the murine retinaEffects of PGF and VEGF inhibition on microgliosis in retinal flat mounts. a Representative images of Iba1-stained microglia/macrophages in single laser spots of retinal flat mounts 3 and 7 days after laser coagulation. Scale bar: 100 μm. b Quantification of mononuclear phagocytes per laser spot in retinal flat mounts 3 days after laser-induced damage (n = 16 laser spots for IgG, n = 14 laser spots for aVEGF, n = 14 laser spots for aPGF, n = 15 laser spots for aVEGF/aPGF, n = 14 laser spots for aflibercept). c Quantification of mononuclear phagocytes per laser spot in retinal flat mounts 7 days after laser-induced damage (n = 14 laser spots for IgG, n = 14 laser spots for aVEGF, n = 15 laser spots for aPGF, n = 27 laser spots for aVEGF/aPGF, n = 28 laser spots for aflibercept). d Quantification of Iba1 signals 3 days after laser coagulation in retinal flat mounts by counting the mean of colored pixels per image (n = 16 laser spots for IgG, n = 14 laser spots for aVEGF, n = 14 laser spots for aPGF, n = 15 laser spots for aVEGF/aPGF, n = 14 laser spots for aflibercept). e Quantification of Iba1 signals 7 days after laser coagulation in retinal flat mounts by counting the mean of colored pixels per image (n = 14 laser spots for IgG, n = 14 laser spots for aVEGF, n = 15 laser spots for aPGF, n = 27 laser spots for aVEGF/aPGF, n = 28 laser spots for aflibercept). f Interleukin 6 (IL-6) levels in retinal flat mounts 6 h after laser damage quantified by ELISA (n = 8 flat mounts per group). Naive (not lasered) animals were used as controls. g Interleukin 1β (IL-1β) levels in retinal flat mounts 6 h after laser damage quantified by ELISA (n = 8 flat mounts per group). h Tumor necrosis factor (TNF) levels in retinal flat mounts 6 h after laser damage quantified by ELISA (n = 8 flat mounts per group). Data are shown as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001)Back to article page