Fig. 7

Characterization of the retinal phenotype after transient ischemia with or without XBD173 treatment. a Central retinal slices were stained with DAPI to visualize the nuclei. b Loss of retinal cells (n = 4–8 animals per group) in the different layers was quantified based on DAPI staining 7 and 14 days post-surgery (dps). c The thickness of the inner and outer plexiform layer (IPL and OPL) was measured in four central retinal slices from each retina. Bars represent values ± SEM from n = 3–4 of each treatment group. d Calretinin stainings (right) of the central retinal slices were used to quantify the survival rates (left) of ganglion and displaced amacrine cells (ganglion cell layer, GCL) and amacrine cells in the inner nuclear layer (INL). Bars represent values ± SEM from n = 3–4 of each treatment group. e Full-field electroretinogram recordings from mice 14 dps show a reduced responsiveness of retinae from XBD173-treated mice already in the control eyes. The relative reduction in light responsiveness after transient ischemia was similar in both treatment groups (n = 8 per group). Whiskers indicate the standard deviation. Scale bars, 20 μm