Fig. 4

Hypercapnia increased the expression of TJ proteins and PKCε. a, b Representative Western blot results showing the expression of the tight junction proteins ZO-1, occludin, and claudin-5 (a) and PKCα, PKCβII, and PKCε (b) in the sham group, group T, group T+H, group T+H+PKCi, and group T+H+PKCαβ rats at 48-h post-FPI. c–h Quantification of the expression of ZO-1 (c), occludin (d), claudin-5 (e), PKCα (f), PKCβII (g), and PKCε (h) in the sham group, group T, group T+H, group T+H+PKCi, and group T+H+PKCαβi rats. i RT-PCR results showing the mRNA expression of PKCε in the sham group, group T, group T+H, group T+H+PKCi, and group T+H+PKCαβI rats. Data were expressed as means ± SEM. n = 4. ^#p < 0.05 vs. sham, *p < 0.05 vs. group T, ^&p < 0.05 vs. group T+H