Iron accentuated reactive oxygen species release by NADPH oxidase in activated microglia contributes to oxidative stress in vitro

Table 2 Cytokine and chemokine responses

Protein	Control Mean ± SEM	FeSO₄ Mean ± SEM	LPS Mean ± SEM	LPS & FeSO₄ Mean ± SEM
G-CSF	ND	ND	0.074 ± 0.013	0.067 ± 0.008
sICAM-1	0.690 ± 0.254	0.813 ± 0.32	0.813 ± 0.324	0.698 ± 0.26
IL-1α	0.171 ± 0.018	0.179 ± 0.021	0.486 ± 0.069	0.47 ± 0.053
IL-1β	ND	ND	0.183 ± 0.023	0.177 ± 0.027
IL-1ra	0.934 ± 0.052	0.87 ± 0.059	1.165 ± 0.023	1.18 ± 0.104
IP-10	0.175 ± 0.042	0.143 ± 0.026	0.365 ± 0.011	0.362 ± 0.021
M-CSF	0.097 ± 0.01	0.107 ± 0.005	0.085 ± 0.004	0.083 ± 0.011
JE	0.573 ± 0.05	0.535 ± 0.055	0.619 ± 0.049	0.568 ± 0.062
MIP-1α	0.941 ± 0.016	1.07 ± 0.115	0.849 ± 0.064	0.769 ± 0.019
MIP-1β	0.023 ± 0.011	0.021 ± 0.012	0.113 ± 0.028	0.106 ± 0.024
MIP-2	ND	ND	0.072 ± 0.01	0.072 ± 0.002
RANTES	0.079 ± 0.005	0.081 ± 0.009	0.186 ± 0.019	0.206 ± 0.008
SDF-1	0.039 ± 0.008	0.038 ± 0.007	0.04 ± 0.009	0.036 ± 0.006
TIMP-1	0.052 ± 0.0007	0.057 ± 0.01	0.058 ± 0.0029	0.063 ± 0.003
TNFα	0.067 ± 0.005	0.074 ± 0.011	0.241 ± 0.038	0.214 ± 0.014
TREM-1	0.08 ± 0.002	0.08 ± 0.007	0.103 ± 0.003	0.091 ± 0.008

Values represent relative expression of cytokines and chemokines normalized to manufacturer’s controls after stimulation. No significant differences were noted between control and FeSO₄ or between LPS and FeSO4 & LPS groups. All groups were compared using two-way ANOVA with Tukey’s post-test. All groups represent N = 3. All data presented as mean ± SEM

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