Fig. 4

Effect of LPS injection on the number of Iba1⁺ cells and bone marrow-derived cells in the SFO. Cryosections were immunostained with antibodies against the myeloid lineage cell marker Iba1 and the outer basement membrane marker laminin-111. a We sacrificed mice every day after LPS injection and found an increase of Iba1⁺ cells 4 days after LPS injection. b Iba1⁺ cells were often seen in the SFO perivascular space of vehicle-treated mice. The number of these cells was increased inside or spanning the laminin-111⁺ outer basement membrane 4 days after LPS injection. c Quantitative analysis revealing the effects of LPS on the number of Iba1⁺ cells in the SFO of adult mice. N = 4. *P < 0.05, determined by Student’s t test. d Experimental design of bone marrow-derived cell transplantation. Mice were injected with busulfan three times (every second day; solid arrows) for immunosuppression prior to bone marrow-derived cell transplantation (open arrow). One month later, busulfan-treated chimeras were injected with vehicle or LPS (solid arrowhead) and sacrificed 4 days later (open arrowhead). e EGFP⁺ cells were often seen in the SFO of vehicle-treated mice. LPS injection increased the number of these cells inside or spanning the laminin-111⁺ outer basement membrane. f Quantitative analysis revealing the effects of LPS on the number of EGFP⁺ cells in the SFO of adult mice. N = 4. *P < 0.05, determined by Student’s t test. LPS (vehicle)-1, − 2, − 3, or − 4 days, 1, 2, 3, or 4 days after LPS (vehicle) injection; Laminin, laminin-111. Scale bars are 10 μm