Figure 4

ALX treatment alleviates the severity of EAE in mice. C57BL/6 mice were injected with MOG35-55 in 50% CFA to induc e EAE and the mice were randomized and treated with vehicle or ALX (from day 0). We measured a the mean clinical scores of each group of mice; b the changes in their body weights; c the cumulative scores of disease severity; d the EAE onset days were calculated from the immunization day to the day of EAE clinical manifestation beginning; e, f the mean pathological scores for inflammation and demyelination in vehicle and ALX-treated mice were measured by semi-quantitative analysis; g the HE analysis of the spinal cord shows the infiltration of inflammatory cells in the white matter. The arrows indicate the inflammatory cells. h LFB analysis of the spinal cord shows the areas of intact myelin (blue) and demyelination (white). The arrows indicate the demyelination in the spinal cord. i Electron microscopic analysis of the lumbar enlargement. The arrows indicate the loose and loss of myelin in the spinal cord. j Immunofluorescent analysis of anti-F4/80 stained macrophages and microglia in the lumbar spinal cord tissue sections from the vehicle-treated and ALX-treated mice. Data are representative images or expressed as the means ± SEM of each group (n = 12). ^※p < 0.05, *p < 0.01, ^#p < 0.001