Fig. 3

FTY720 attenuates the severity of alterations occurring in sciatic nerves of c-EAN rats. Representative semi-thin cross-sections of sciatic nerves from control and c-EAN rats treated with FTY720 or vehicle (three groups of rats) and stained with toluidine blue at a 18 dpi and b 60 dpi. c Number of myelinated fibers per square millimeter at 18 and 60 dpi and d percentage of small (perimeter < 19 μm) and large (perimeter > 19 μm) myelinated fibers at 60 dpi in sciatic nerves from control and c-EAN rats treated with FTY720 or vehicle. Mean values, SEM, and p values are indicated. *p < 0.05; **p < 0.01. n, number of rats. Representative paraffin cross sections of sciatic nerves taken from the three groups of rats and labeled with e anti-MBP antibody for myelin and f anti-SMI-31 antibody for phosphorylated neurofilament at 60 dpi