Fig. 4

Orexin A strikingly diminishes demyelinating processes, astrogliosis, and microglial/macrophage responses in the CNS. On day 21 post-immunization, spinal cord sections from naïve, PBS, and OxA groups were prepared and labeled with a Alexa 488 anti-MBP or Alexa 488 anti-GFAP, and b DAPI, Cy3 anti-Iba-1, and Alexa 488 anti-Arg-1 stainings. All images were taken at × 20 magnification. Two representative areas of spinal cord are shown per experimental group. Graphs show the percentage of MBP, GFAP, and Iba1 immunoreactive areas as well as the Arg-1-positive cell number per mm² of each group (n = 7/group). *P < 0.05 (Mann-Whitney test, compared to PBS group). ND not detectable