Fig. 6

Orexin A does not interfere MOG-induced proliferation and cytokine release in lymph node cultures from PBS- and OxA-treated EAE mice. At the peak of the disease, on day 15 post-MOG immunization, draining lymph node cells from PBS- and OxA-treated mice were stimulated ex vivo with 20 μg/mL of MOG_35–55 peptide or ovalbumin (Ova) in the presence or absence of OxA (1 × 10⁻⁶ M). a Shows cell proliferation that was measured by [³H]-thymidine incorporation. b Shows proinflammatory cytokine (i.e., IFN-γ and IL-17) secretion into the culture supernatant measured by ELISA. In both groups, all parameters measured were. No statistical differences were found between PBS and Orexin A treatments (Mann-Whitney test)