Fig. 4

Concentration-dependence and kinetics of atorvastatin upregulation of astrocyte CD55 expression. a Atorvastatin concentration-dependence in U-251MG cells following 24-h incubation. Representative immunofluorescence shown at the left and summary at the right (mean ± SEM, n = 7, *P < 0.05, **P < 0.01, unpaired Student’s t test, compared with DMSO). b Time course of CD55 upregulation following incubation with 5 μM atorvastatin. Representative immunofluorescence shown at the left and summary at the right (mean ± SEM, n = 7, *P < 0.05, **P < 0.01, unpaired Student’s t test, compared with 0 h). c RT-PCR of CD55 and β-actin transcript expression following 48-h incubation of U-251MG cells with 1 μM atorvastatin. d CD55 immunofluorescence in U-251MG cell following 24-h incubation with 1 μM atorvastatin in the absence or presence of 3 μg/mL cycloheximide. Representative of two sets of experiments