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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Visualizing neuroinflammation with fluorescence and luminescent lanthanide-based in situ hybridization

Fig. 2

Toll-like receptor 4 (TLR4) mRNA staining in BV2 microglia cells and J774 macrophage cells (biotinylated TLR4 cRNA probe conjugated to streptavidin (SA)-Alexafluor555) following 0 h and 4 h of LPS stimulation (0.5 μg/ml) compared to cellular autofluorescence. a DAPI (blue) and BV2 cell autofluorescence or TLR4 mRNA expression (orange) following LPS-induced inflammation. b DAPI (blue) and J774 cell autofluorescence or TLR4 mRNA expression (orange) following LPS-induced inflammation. c Brightness intensities of LPS-treated BV2 cells represented in arbitrary fluorescence units. d Brightness intensities of LPS-treated J774 cells represented in arbitrary fluorescence units. 40x magnification, scale bar = 20 μm

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Journal of Neuroinflammation

ISSN: 1742-2094