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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Visualizing neuroinflammation with fluorescence and luminescent lanthanide-based in situ hybridization

Fig. 5

Fluorescence and luminescent lanthanide detection in L5 mouse spinal cords from sham-operated and CCI pain treatment mice are shown. a, b Sham and CCI pain animal spinal cord luminescence using only SA-BHHTEGST-Eu3+ chelate staining (a; without probe) and time-gated microscopy (b; weak area of SA-BHHTEGST-Eu3+ chelate background fluorescence indicated by arrow). c, d Sham or CCI pain animal spinal cord luminescence using LISH-based TLR4 mRNA staining and time-gated microscopy (d; strong region of TLR4 mRNA staining indicated by arrow). e, f Sham or CCI pain animal spinal cord luminescence using FISH (SA-Alexafluor488)-based TLR4 mRNA staining. g, h Sham or CCI pain animal spinal cord luminescence using GFAP immunofluorescence (IF), arrows indicate regions positive for GFAP protein. Scale bars = 100 μm

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